TY - JOUR AB - Increasing energy demands, decreasing fossil fuel resources, instability of crude oil prices and pollution problems have compelled to switch over bio-based fuel for transportation, for example, biodiesel. In present study, waste cooking oil (WCO) was evaluated as feedstock for biodiesel production using free lipase in liquid. The response surface methodology (RSM) was used to optimize the interaction between four factors: the reaction temperature, methanol-oil molar ratio, dosage of lipase as biocatalyst and rotational speed. Using this method, the model predicted the optimal conditions reaching up to 86% FAME yield with temperature 44.2 degrees C, methanol-oil molar ratio (3.05:1), amount of lipase 0.782 g and rotation speed of 170 rpm with incubation period of 24 h. The reactions carried out under optimized conditions confirmed the validity of the model. The use of WCO in lipase catalyzed process can diminish the cost of biodiesel production and will allow lowering the direct use of edible oils to produce biodiesel instead. Thus, present study shows great practical potential to replace fossil fuel with renewable fuel (biodiesel). (C) 2017 Elsevier Ltd. All rights reserved. AD - East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China AN - WOS:000400212500009 AU - Ali, C. H. AU - Qureshi, A. S. AU - Mbadinga, S. M. AU - Liu, J. F. AU - Yang, S. Z. AU - Mu, B. Z. DA - Aug DO - 10.1016/j.renene.2017.03.018 J2 - Renew Energ KW - biodiesel LA - English N1 - Et3xh PY - 2017 SN - 0960-1481 SP - 93-100 ST - Biodiesel production from waste cooking oil using onsite produced purified lipase from Pseudomonas aeruginosa FW_SH-1: Central composite design approach T2 - Renewable Energy TI - Biodiesel production from waste cooking oil using onsite produced purified lipase from Pseudomonas aeruginosa FW_SH-1: Central composite design approach UR - ://WOS:000400212500009 VL - 109 ID - 13499 ER -