TY - JOUR AB - The transesterification of coconut oil with ethanol catalyzed by Burkholderia cepacia lipase immobilized on polysiloxane-polyvinyl alcohol was performed in a continuous flow. The experimental design consisted of a two-stage packed-bed reactor incorporating a column with cationic resin (Lewatit GF 202) to remove the glycerol formed as by-product and the reactor performance was quantified for three different flow rates corresponding to space-times from 10 to 14 h. The influence of space-time on the ethyl ester (FAEE) concentrations, yields and productivities was determined. The reactor operation was demonstrated for space-time of 14 h attaining FAEE concentrations of 58.5 +/- 0.87 wt%, FAEE yields of 97.3 +/- 1.9 % and productivities of 41.6 +/- 1.0 mg(ester) g (medium) (-1) h(-1). Biodiesel purified samples showed average kinematic viscosity values of 5.5 +/- 0.3 mm(2) s(-1) that meet the criteria established by the American National Standard ASTM (D6751). The immobilized lipase was found to be stable regarding its morphological and catalytic characteristics, showing half-life time (t (1/2)) around 1540 h. The continuous packed-bed reactor connected in series with simultaneous glycerol removal has a great potential to attain high level of transesterification yields, raising biodiesel productivity. AD - Univ Sao Paulo, Engn Sch Lorena, BR-12602810 Sao Paulo, Brazil AN - WOS:000382117800011 AU - Silva, W. C. E. AU - Freitas, L. AU - Oliveira, P. C. AU - de Castro, H. F. DA - Oct DO - 10.1007/s00449-016-1636-3 IS - 10 J2 - Bioproc Biosyst Eng KW - biodiesel LA - English N1 - Du3nh PY - 2016 SN - 1615-7591 SP - 1611-1617 ST - Continuous enzymatic biodiesel production from coconut oil in two-stage packed-bed reactor incorporating an extracting column to remove glycerol formed as by-product T2 - Bioprocess and Biosystems Engineering TI - Continuous enzymatic biodiesel production from coconut oil in two-stage packed-bed reactor incorporating an extracting column to remove glycerol formed as by-product UR - ://WOS:000382117800011 VL - 39 ID - 13203 ER -