TY - JOUR AB - Whole cell lipase catalysis and microalgal feedstocks make overall biodiesel synthesis greener and sustainable. In this study, a novel approach of whole cell lipase-catalyzed conversion of Scenedesmus obliquus lipids was investigated for biodiesel synthesis. Microalgal biodiesel was characterized for its fuel properties. Optimization of process parameters for immobilized Aspergillus niger whole cell lipase-catalyzed biodiesel synthesis was carried out. Highest biodiesel conversion of 53.76% was achieved from S. obliquus lipids at 35 degrees C, methanol to oil ratio of 5:1 and 2.5% water content based on oil weight with 6 BSPs (Biomass support particles). Step-wise methanol addition was applied to account for methanol tolerance, which improved biodiesel conversion upto 80.97% and gave 90.82 +/- 1.43% yield. Immobilized A. niger lipase can be used for 2 batches without significant loss in conversion efficiency. Most of the fuel properties of biodiesel met the specifications set by international standards. (c) 2015 Elsevier Ltd. All rights reserved. AD - Durban Univ Technol, Inst Water & Wastewater Technol, Durban, South Africa AN - WOS:000363344800098 AU - Guldhe, A. AU - Singh, P. AU - Kumari, S. AU - Rawat, I. AU - Permaul, K. AU - Bux, F. DA - Jan DO - 10.1016/j.renene.2015.07.059 J2 - Renew Energ KW - whole cell lipase LA - English N1 - Cu2ho PY - 2016 SN - 0960-1481 SP - 1002-1010 ST - Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst T2 - Renewable Energy TI - Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst UR - ://WOS:000363344800098 VL - 85 ID - 12516 ER -