TY - JOUR AB - BACKGROUND: Photoautotrophic microalgae are a promising avenue for sustained biodiesel production, but are compromised by low yields of biomass and lipids at present. We are developing a chemical approach to improve microalgal accumulation of feedstock lipids as well as high-value alpha-linolenic acid which in turn might provide a driving force for biodiesel production. RESULTS: We demonstrate the effectiveness of the small bioactive molecule "acetylcholine" on accumulation of biomass, total lipids, and alpha-linolenic acid in Chlorella sorokiniana. The effectiveness exists in different species of Chlorella. Moreover, the precursor and analogs of acetylcholine display increased effectiveness at higher applied doses, with maximal increases by 126, 80, and 60% over controls for biomass, total lipids, and alpha-linolenic acid, respectively. Production of calculated biodiesel was also improved by the precursor and analogs of acetylcholine. The biodiesel quality affected by changes in microalgal fatty acid composition was addressed. CONCLUSION: The chemical approach described here could improve the lipid yield and biodiesel production of photoautotrophic microalgae if combined with current genetic approaches. AD - Laboratory of Biosystems Engineering, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014 Jiangsu China. AN - 25688288 AU - Parsaeimehr, A. AU - Sun, Z. AU - Dou, X. AU - Chen, Y. F. C2 - PMC4329656 DO - 10.1186/s13068-015-0196-0 KW - Acetylcholine N1 - Parsaeimehr, Ali PY - 2015 SN - 1754-6834 (Print) SP - 11 ST - Simultaneous improvement in production of microalgal biodiesel and high-value alpha-linolenic acid by a single regulator acetylcholine T2 - Biotechnol Biofuels TI - Simultaneous improvement in production of microalgal biodiesel and high-value alpha-linolenic acid by a single regulator acetylcholine UR - https://www.ncbi.nlm.nih.gov/pubmed/25688288 VL - 8 ID - 15350 ER -