TY - JOUR AB - The production of technical-grade sodium citrate from the glycerol-containing biodiesel waste by Yarrowia lipolytica was studied. Batch experiments showed that citrate was actively produced within 144 h, then citrate formation decreased presumably due to inhibition of enzymes involved in this process. In contrast, when the method of repeated batch cultivation was used, the formation of citrate continued for more than 500 h. In this case, the final concentration of citrate in the culture liquid reached 79-82 g/L. Trisodium citrate was isolated from the culture liquid filtrate by the addition of a small amount of NaOH, so that the pH of the filtrate increased to 7-8. This simple and economic isolation procedure gave the yield of crude preparation containing trisodium citrate 5.5-hydrate up to 82-86%. (C) 2015 Elsevier Ltd. All rights reserved. AD - Russian Acad Sci, GK Skryabin Inst Biochem & Microorganisms, Pushchino 142290, Moscow Region, Russia AN - WOS:000358380900034 AU - Kamzolova, S. V. AU - Vinokurova, N. G. AU - Lunina, J. N. AU - Zelenkova, N. F. AU - Morgunov, I. G. DA - Oct DO - 10.1016/j.biortech.2015.06.092 J2 - Bioresource Technol KW - yarrowia lipolytica yeast LA - English N1 - Cn4dt PY - 2015 SN - 0960-8524 SP - 250-255 ST - Production of technical-grade sodium citrate from glycerol-containing biodiesel waste by Yarrowia lipolytica T2 - Bioresource Technology TI - Production of technical-grade sodium citrate from glycerol-containing biodiesel waste by Yarrowia lipolytica UR - ://WOS:000358380900034 VL - 193 ID - 11379 ER -