TY - JOUR AB - The obtaining of Ecodiesel, a biofuel applicable to diesel engines which keeps the glycerin as monoglyceride (MG), was achieved through a selective ethanolysis process of sunflower oil, by application of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on macroporous anion exchange resins. This biocatalyst that was already described in the synthesis of conventional biodiesel has also shown its efficiency in the present selective enzymatic process, after optimization of the influence of various reaction parameters. Thus, an adequate activity is obtained that is maintained throughout five successive reuses. Quantitative conversions of triglycerides (TG) with high yields to fatty acid ethyl esters (FAEE) were obtained under mild reaction conditions that correspond to the transformation of TG in a mixture of two moles of FAEE and a mole of MG, thus avoiding the glycerol production. Thus, the selective transesterification reaction of sunflower oil with absolute ethanol can be carried out under standard conditions with oil/ethanol volume ratio 12/3.5 (mL), at constant pH obtained by the addition of 50 mu l of aqueous solution of 10 N NaOH, reaction temperature of 40 degrees C and 40 mg of Lipozyme RM IM. Under these experimental conditions six successive reactions can be efficiently carried out. AD - Univ Cordoba, Dept Organ Chem, Cordoba 14014, Spain AN - WOS:000341316100010 AU - Calero, J. AU - Verdugo, C. AU - Luna, D. AU - Sancho, E. D. AU - Luna, C. AU - Posadillo, A. AU - Bautista, F. M. AU - Romero, A. A. DA - Dec 25 DO - 10.1016/j.nbt.2014.02.008 IS - 6 J2 - New Biotechnol KW - pig pancreatic lipase LA - English N1 - Ao4nh PY - 2014 SN - 1871-6784 SP - 596-601 ST - Selective ethanolysis of sunflower oil with Lipozyme RM IM, an immobilized Rhizomucor miehei lipase, to obtain a biodiesel-like biofuel, which avoids glycerol production through the monoglyceride formation T2 - New Biotechnology TI - Selective ethanolysis of sunflower oil with Lipozyme RM IM, an immobilized Rhizomucor miehei lipase, to obtain a biodiesel-like biofuel, which avoids glycerol production through the monoglyceride formation UR - ://WOS:000341316100010 VL - 31 ID - 9761 ER -