TY - JOUR AB - Biodiesel, a non-toxic and biodegradable fuel, has recently become a major source of renewable alternative fuels. Utilization of lipase as a biocatalyst to produce biodiesel has advantages over common alkaline catalysts such as mild reaction conditions, easy product separation, and use of waste cooking oil as raw material. In this study, Pseudomonas cepacia lipase immobilized onto magnetic nanoparticles (MNP) was used for biodiesel production from waste cooking oil. The optimal dosage of lipase-bound MNP was 40% (w/w of oil) and there was little difference between stepwise addition of methanol at 12 h- and 24 h-intervals. Reaction temperature, substrate molar ratio (methanol/oil), and water content (w/w of oil) were optimized using response surface methodology (RSM). The optimal reaction conditions were 44.2 degrees C, substrate molar ratio of 5.2, and water content of 12.5%. The predicted and experimental molar conversions of fatty acid methyl esters (FAME) were 80% and 79%, respectively. AD - Tatung Univ, Dept Bioengn, Taipei 10452, Taiwan AN - WOS:000330219800058 AU - Yu, C. Y. AU - Huang, L. Y. AU - Kuan, I. C. AU - Lee, S. L. DA - Dec DO - 10.3390/ijms141224074 IS - 12 J2 - Int J Mol Sci KW - biodiesel LA - English N1 - 296xx PY - 2013 SN - 1422-0067 SP - 24074-24086 ST - Optimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic Nanoparticles T2 - International Journal of Molecular Sciences TI - Optimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic Nanoparticles UR - ://WOS:000330219800058 VL - 14 ID - 9516 ER -