TY - JOUR AB - Cultivation in glycerol instead of sugars inhibits 2,3-butanediol (2,3-BD) production by Bacillus amyloliquefaciens. In this study, we report that B. amyloliquefaciens readily produces 2,3-BD from biodiesel-derived glycerol in the presence of beet molasses as a co-substrate. Unexpectedly, the molasses stimulated 2,3-BD production and simultaneously reduced the duration of fermentation. Productivity of 2,3-BD was enhanced at the start of fermentation, and yields increased under continuous molasses supply. Subsequently, 2,3-BD production in molasses-supplemented fed-batch culture was observed. Prior to inoculation of fed-batch fermentation culture, 15 g/l of molasses was added to the bioreactor. After 6 h of incubation, the bioreactor was fed with a solution containing 80 % glycerol and 15 % molasses. The 2,3-BD concentration, yield, and productivity significantly improved, reaching 83.3 g/l, 0.42 g/g, and 0.87 g/l center dot h, respectively. To our knowledge, these results are the highest report for 2,3-BD fermentation from biodiesel-derived glycerol. AD - Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China AN - WOS:000323281900012 AU - Yang, T. W. AU - Rao, Z. M. AU - Zhang, X. AU - Xu, M. J. AU - Xu, Z. H. AU - Yang, S. T. DA - Sep DO - 10.1007/s00253-013-5048-x IS - 17 J2 - Appl Microbiol Biot KW - 2,3-butanediol LA - English N1 - 203he PY - 2013 SN - 0175-7598 SP - 7651-7658 ST - Fermentation of biodiesel-derived glycerol by Bacillus amyloliquefaciens: effects of co-substrates on 2,3-butanediol production T2 - Applied Microbiology and Biotechnology TI - Fermentation of biodiesel-derived glycerol by Bacillus amyloliquefaciens: effects of co-substrates on 2,3-butanediol production UR - ://WOS:000323281900012 VL - 97 ID - 9497 ER -