TY - JOUR AB - The effect of the composition of the feedstock (free fatty acids and methanol), the presence or absence of moisture and the contact with biodiesel on the catalytic, enzymatic and physical stability of Novozym (R) 435 during enzymatic biodiesel production was studied. The continuous removal of moisture leads to the deactivation of the biocatalyst and favours the degradation of the polymethylmethacrylate (PMMA) that constitutes the support of the lipase B of Candida antarctica (CALB). Nevertheless, the presence of PMMA along with the active protein was detected in the reaction media regardless of the operating conditions. Surprisingly, biodiesel (without the presence of methanol) is able to diffuse inside the biocatalyst's beads producing its swelling, modifying the internal texture and also dissolving the polymeric matrix. (C) 2013 Elsevier B. V. All rights reserved. AD - Univ Nacl La Plata, CONICET, Ctr Invest & Desarrollo Ciencias Aplicada Dr Jorg, Buenos Aires, DF, Argentina AN - WOS:000321748000011 AU - Jose, C. AU - Austic, G. B. AU - Bonetto, R. D. AU - Burton, R. M. AU - Briand, L. E. DA - Sep 15 DO - 10.1016/j.cattod.2013.02.013 J2 - Catal Today KW - novozym (r) 435 LA - English N1 - 182mu PY - 2013 SN - 0920-5861 SP - 73-80 ST - Investigation of the stability of Novozym (R) 435 in the production of biodiesel T2 - Catalysis Today TI - Investigation of the stability of Novozym (R) 435 in the production of biodiesel UR - ://WOS:000321748000011 VL - 213 ID - 8822 ER -