TY - JOUR AB - To improve enzymatic biodiesel production, we developed a packed-bed reactor (PBR) system using recombinant cells, into which a strong enolase promoter and 5' untranslated region were introduced. Aspergillus oryzae expressing Fusarium heterosporum lipase was immobilized within biomass support particles (BSPs) during cultivation and used directly as a whole-cell biocatalyst. BSP-immobilized A. oryzae carrying three copies of the expression cassette showed a higher performance than previously developed cell systems, resulting in 87.5% conversion after 10 passes in PBR. The performance was also affected by operational factors including residence time and methanol feeding. After optimization, the PBR system attained a final methyl ester content of 96.1% with a residence time of 140 min per pass and stepwise addition of 4.25 molar equivalents of methanol to oil for 6 passes. Moreover, lipase activity was maintained for 5 batch cycles. Therefore, the developed PBR employing a high-lipase-expression system is considered useful for improving enzymatic biodiesel production. (C) 2011 Elsevier B.V. All rights reserved. AD - Kobe Univ, Dept Sci & Chem Engn, Grad Sch Engn, Nada Ku, Kobe, Hyogo 6578501, Japan AN - WOS:000303181000010 AU - Yoshida, A. AU - Hama, S. AU - Tamadani, N. AU - Fukuda, H. AU - Kondo, A. DA - Apr 15 DO - 10.1016/j.bej.2011.11.003 J2 - Biochem Eng J KW - biodiesel fuel LA - English N1 - 930yo PY - 2012 SN - 1369-703x SP - 76-80 ST - Improved performance of a packed-bed reactor for biodiesel production through whole-cell biocatalysis employing a high-lipase-expression system T2 - Biochemical Engineering Journal TI - Improved performance of a packed-bed reactor for biodiesel production through whole-cell biocatalysis employing a high-lipase-expression system UR - ://WOS:000303181000010 VL - 63 ID - 8309 ER -