TY - JOUR AB - A strain of Aspergillus niger isolated from atmospherically exposed bread and Jatropha curcas seed was utilized as a whole-cell biocatalyst for palm oil methanolysis to produce fatty acid methyl esters (FAME), or biodiesel. The A. niger strain had a lipase activity of 212.58 mU mL(-1) after 144 h incubation at 25 degrees C with an initial pH value of 6.5, using 7% polypeptone (w/w on basal medium) as the nitrogen source and 3% olive oil (w/w on basal medium) as a carbon source. The A. niger cells spontaneously immobilized within polyurethane biomass support particles (BSPs) during submerged fermentation. Thereafter, the methanolysis of palm oil was achieved via a three-step addition of methanol in the presence of BSPs-immobilized with A. niger cells. The influence of water content, reaction temperature and enzyme concentration on reaction rate was investigated. An 8% water content and a temperature of 40 degrees C in the presence of 30 immobilized BSPs, resulted in an 87% FAME yield after 72 h. AD - Natl Univ Singapore, Div Environm Sci & Engn, Singapore 117576, Singapore AN - WOS:000278038000001 AU - Xiao, M. AU - Mathew, S. AU - Obbard, J. P. DA - Apr DO - 10.1111/j.1757-1707.2010.01038.x IS - 2 J2 - Gcb Bioenergy KW - activity LA - English N1 - 601dj PY - 2010 SN - 1757-1693 SP - 45-51 ST - A newly isolated fungal strain used as whole-cell biocatalyst for biodiesel production from palm oil T2 - Global Change Biology Bioenergy TI - A newly isolated fungal strain used as whole-cell biocatalyst for biodiesel production from palm oil UR - ://WOS:000278038000001 VL - 2 ID - 6097 ER -