TY - JOUR AB - The production of biodiesel was investigated using a lipase-catalyzed (Novozym 435) reaction involving methyl acetate and ionic liquid [BMIM][PF6] as a co-solvent. The use of a lipase catalyst removed the need for alkali wastewater removal and treatment, and the methyl acetate and ionic liquid helped prevent deactivation and improve activity and stability of the lipase, respectively. Experiments were carried out in shake flask and jacketed conical reactors. Runs performed in shake flask reactor produced similar fatty acid methyl ester (FAME) yields to previous work using a small-scale reactor (83%), indicating that the reaction can achieve high yields provided that sufficient mixing between the oil and ionic liquid phases occur. The highest yield achieved in the conical reactor was 54% because of the relatively poor mixing in the reactor as a result of two phases present in the mixture. An additional benefit of using ionic liquid as a co-solvent in the process was for its ease of separation of products. Two distinct phases were present at the end of the reaction, with the ionic liquid phase containing the triacetylglycerol byproduct and any unreacted methyl acetate. The byproduct was then separated by washing with water, and the ionic liquid was removed by decantation and reused. FAMEs were also successfully produced from waste canola oil, with a 72% yield achieved in a small-scale reactor and a 30% yield in a 500 mL jacketed conical reactor. AD - Univ Western Ontario, Fac Engn, Dept Chem & Biochem Engn, London, ON N6A 5B9, Canada AN - WOS:000278851000053 AU - Ruzich, N. I. AU - Bassi, A. S. DA - May DO - 10.1021/ef901428k J2 - Energ Fuel KW - candida-antarctica lipase LA - English N1 - 611ve PY - 2010 SN - 0887-0624 SP - 3214-3222 ST - Investigation of Lipase-Catalyzed Biodiesel Production Using Ionic Liquid [BMIM][PF6] as a Co-solvent in 500 mL Jacketed Conical and Shake Flask Reactors Using Triolein or Waste Canola Oil as Substrates T2 - Energy & Fuels TI - Investigation of Lipase-Catalyzed Biodiesel Production Using Ionic Liquid [BMIM][PF6] as a Co-solvent in 500 mL Jacketed Conical and Shake Flask Reactors Using Triolein or Waste Canola Oil as Substrates UR - ://WOS:000278851000053 VL - 24 ID - 5893 ER -