TY - JOUR AB - The methanolysis of soybean oil to produce a fatty acid methyl ester (ME, i.e., biodiesel fuel) was catalyzed by lipase-producing filamentous fungi immobilized on biomass support particles (BSPs) as a whole-cell biocatalyst in the presence of ionic liquids. We used four types of whole-cell biocatalysts: wildtype Rhizopus oryzae producing triacylglycerol lipase (w-ROL), recombinant Aspergillus oryzae expressing Fusarium heterosporum lipase (r-FHL), Candida antarctica lipase B (r-CALB), and mono- and diacylglycerol lipase from A. oryzae (r-mdlB). w-ROL gave the high yield of fatty acid methyl ester (ME) in ionic liquid [Emim][BF(4)] or [Bmim][BF(4)] biphasic systems following a 24h reaction. While lipases are known to be severely deactivated by an excess amount of methanol (e.g. 1.5 Mequiv. of methanol against oil) in a conventional system, methanolysis successfully proceeded even with a methanol/oil ratio of 4 in the ionic liquid biphasic system, where the ionic liquids would work as a reservoir of methanol to suppress the enzyme deactivation. When only w-ROL was used as a biocatalyst for methanolysis, unreacted monoglyceride remained due to the 1,3-positional specificity of R. oryzae lipase. High ME conversion was attained by the combined use of two types of whole-cell biocatalysts, w-ROL and r-mdlB. In a stability test, the activity of w-ROL was reduced to one-third of its original value after incubation in [Bmim][BF(4)] for 72 h. The stability of w-ROL in [Bmim][BF(4)] was greatly enhanced by cross-linking the biocatalyst with glutaraldehyde. The present Study demonstrated that ionic liquids are promising candidates for use as the second solvent in biodiesel fuel production by whole-cell biocatalysts. (C) 2009 Elsevier Inc. All rights reserved. AD - Kobe Univ, Grad Sch Engn, Dept Chem Sci & Engn, Nada Ku, Kobe, Hyogo 6578501, Japan AN - WOS:000272347400009 AU - Arai, S. AU - Nakashima, K. AU - Tanino, T. AU - Ogino, C. AU - Kondo, A. AU - Fukuda, H. DA - Jan 7 DO - 10.1016/j.enzmictec.2009.08.008 IS - 1 J2 - Enzyme Microb Tech KW - ionic liquid LA - English N1 - 527dr PY - 2010 SN - 0141-0229 SP - 51-55 ST - Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids T2 - Enzyme and Microbial Technology TI - Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids UR - ://WOS:000272347400009 VL - 46 ID - 5258 ER -